Skip to main content
Figure 4 | Molecular Cancer

Figure 4

From: Human homolog of Drosophila H airy and e nhancer of s plit 1, Hes1, negatively regulates δ-catenin (CTNND2) expression in cooperation with E2F1 in prostate cancer

Figure 4

Overexpression of Hes1 suppressed the δ-catenin expression. (A) Western blots showing the expression of δ-catenin, Hes1, and actin in total lysates of CWR22-Rv1 cells transfected with WT-Hes1, DN-Hes1 or pcDNA3 (vector as control), respectively. (B) RT-PCR analysis of the expression of δ-catenin, Hes1, and GAPDH genes in CWR22-Rv1 cells transfected with WT-Hes1, DN-Hes1 or pcDNA3, respectively. (C) Western blots showing the expression of δ-catenin, Hes1, and actin in total lysates of PC3 cells transfected with WT-Hes1, DN-Hes1 or pcDNA3, respectively. (D) RT-PCR analysis of δ-catenin, Hes1, and GAPDH gene expression in PC3 cells transfected with WT-Hes1, DN-Hes1 or pcDNA3, respectively. Note: Actin or GAPDH as either Western blot or RT-PCR control. The numbers beneath each gel lane reflects the relative intensity compared to vector control and normalized against actin or GAPDH (p < 0.05). Both the Western blot and RT-PCR experiments were repeated at least three times.

Back to article page

Molecular Cancer

ISSN: 1476-4598