Figure 5From: Human homolog of Drosophila H airy and e nhancer of s plit 1, Hes1, negatively regulates δ-catenin (CTNND2) expression in cooperation with E2F1 in prostate cancerImmunofluorescence light microscopy of CWR22-Rv1 cells transfected with WT-Hes1, DN-Hes1 or pcDNA3, respectively. Red shows Hes1 protein stained by anti-flag-M5 antibody. Green shows δ-catenin stained by R1152 antibody. Blue shows nuclei stained with Hoechst 33258. To better reveal the cell-cell boundary in otherwise clustered CWR22-Rv1 cells, purple lines were drawn to indicate the cells transfected while the green lines marked the cells untransfected in K and L. Arrows (B, E, H, K and N): cells transfected with WT-Hes1; Arrows (C, F, I, L and O): DN-Hes1. Arrowheads: cells transfected with pcDNA3. Bar: 10 μm.Back to article page