Figure 7

Reduction of Hes1 corresponds to increased δ-catenin expression. (A-D). Inhibition of γ-secretase decreases Hes1 expression while increasing δ-catenin expression. PC3 (A and B) and CWR22-Rv1 (C and D) cells were treated with (DAPT) or without (Control) the γ-secretase inhibitor DAPT at 20 μM for 48 hours. DAPT reduced Hes1 expression and increased δ-catenin expression. (A) Western blot analyses showing Hes1 and δ-catenin expression in PC3 cells with GAPDH as a loading control. (B) RT-PCR analyses for Hes1, E2F1 and δ-catenin in PC3 cells with GAPDH as PCR control. (C) Western blot analyses showing Hes1 and δ-catenin expression in CWR22-Rv1 cells with GAPDH as a loading control. (D) RT-PCR analyses for Hes1, E2F1 and δ-catenin in CWR22-Rv1 cells with GAPDH as PCR control. (E-F). siRNA against Hes1 suppressed Hes1 expression while increasing δ-catenin expression in PC3 cells. (E). siRNA against Hes1 nearly depleted Hes1 expression level compared to scrambled siRNA as a transfection control. (F). siRNA against Hes1 increased δ-catenin expression level by 6-fold compared to scrambled siRNA as a transfection control. KD: Knockdown. Two independent experiments were performed.