Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Deletion or insertion in the first immunoglobulin-plexin-transcription (IPT) domain differentially regulates expression and tumorigenic activities of RON receptor Tyrosine Kinase

Figure 3

Generation of RONp110 from RONE5/6inbut not from RON160 in response to trypsin or cell-derived trypsin-like proteases. A) M-RON, M-RON160 and M-RONE5/6in cells (2 × 106 cells per sample) were incubated for 24 h and then treated with 0.05% of trypsin in the presence or absence of STI in DMEM at 37°C for 5, 15, and 30 min. Proteins (50 μg per sample) from cell lysates were analyzed by Western blot analysis using rabbit anti-RON antibody. B) Cells were incubated for 72 h and then treated at 37°C with different amounts of FBS in DMEM for 15 min. Western blot analysis was performed as described in B. C) Effect of STI on RONp110 formation mediated by cell-associated proteases. M-RONE5/6in cells in serum-free DMEM were treated at 37°C with different amounts of STI for 48 h. Levels of RONp110 in cell lysates were determined by Western blot analysis as detailed in C. Results shown here were from one of three experiments with similar results.

Back to article page

Molecular Cancer

ISSN: 1476-4598