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Figure 4 | Molecular Cancer

Figure 4

From: Deletion or insertion in the first immunoglobulin-plexin-transcription (IPT) domain differentially regulates expression and tumorigenic activities of RON receptor Tyrosine Kinase

Figure 4

Differential conversions of pro-RON160 and pro-RONE5/6inexpressed in MDCK cells. A) Time-dependent conversion of pro-RON160 and pro-RONE5/6in in MDCK cells. Individual cell lines (2 × 106 cells/sample) were treated with 0.05% trypsin for 30 min to eliminate mature β-chain and then incubated with DMEM containing 10% FBS for variable hours. Cell proteins were subjected to Western blot analysis as described above Figure 3. B) Proteolytic effect of furin on pro-RON160 and pro-RONE5/6in. Purified pro-RON, RON160 and RONE5/6in in DMEM were incubated at 37°C with different amounts of furin for 5 h as previously described [37]. The samples were then subjected to Western blot analysis as described above. Protein intensity was determined by VersaDoc Imagining software as previously described [28].

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