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Figure 5 | Molecular Cancer

Figure 5

From: Deletion or insertion in the first immunoglobulin-plexin-transcription (IPT) domain differentially regulates expression and tumorigenic activities of RON receptor Tyrosine Kinase

Figure 5

Accelerated down-regulation of RONE5/6inbut not RON160 upon anti-RON mAb treatment: A) Zt/g4-induced receptor down regulation. M-RON, M-RON160 and M-RONE5/6in cells (2 × 106 cells per sample) were treated at 37°C with 10 μg/ml of Zt/g4 for various intervals. Normal mouse IgG was used as the negative control. Cellular proteins (50 μg/ml per sample) from cell lysates were subjected to Western blot analysis using rabbit anti-RON C-terminus antibody. B) Intensity of individual protein bands from different groups in A were compared and plotted against various time points after Zt/g4 treatment. C) Preventive effect of Ccm A on Zt/g4-induced receptor down-regulation. Cells were treated for 12 h as described above. Ccm A was added after initiation of Zt/g4 treatment followed by Western blot analysis. D) Preventive effect of lactacystin on Zt/g4-inuced receptor down-regulation. Cells were treated for 12 h as described above. Lactacystin was added after initiation of Zt/g4 treatment followed by Western blot analysis. Data shown here are from one of three experiments with similar results.

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