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Figure 7 | Molecular Cancer

Figure 7

From: Deletion or insertion in the first immunoglobulin-plexin-transcription (IPT) domain differentially regulates expression and tumorigenic activities of RON receptor Tyrosine Kinase

Figure 7

Effect of RON, RON160, and RONE5/6in on focus formation and anchorage independent growth: A) Focus formation by transformed NIH3T3 cells. NIH3T3 cells (1 × 105 cells per dish) were transiently transfected with 3 μg of individual pcDNA3 expression vector containing RON, RON160 or RONE5/6in cDNA as previously described [13]. After incubation for 48 h, cells in 2% FBS-DMEM were stimulated with or without 2 nM of MSP for additional 8 days. Focus formation was observed, photographed, and counted from individual groups. B) Colony formation in soft agar by transfected NIH3T3 cells. The soft agar assays were performed as previously described [13]. NIH3T3, 3T3-RON, 3T3-RON160, and 3T3-RONE5/6in cells (1000 cells per dish) were seeded in soft agar with or without 2 nM of MSP for 25 days. Cell growth at ≥30 cells per cluster was marked as a positive colony and counted.

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