Deletion or insertion in the first immunoglobulin-plexin-transcription (IPT) domain differentially regulates expression and tumorigenic activities of RON receptor Tyrosine Kinase

Table 1 Identification of RON mRNA transcripts with alterations in the first IPT Unit in colon, breast, and pancreatic cancer cells.

		Types of RON mRNA transcripts*
Cancer Cell lines	Tissue sources	Wild type (0.54 kb)	Deletion of exons 5 & 6 (0.21 kb)	Insertion btw exons 5 & 6 (0.6 kb)
HT-29	Colon	+	+	+
SW620	Colon	+	+	+
HCT116	Colon	+	-	-
SW837	Colon	+	+	-
T-47D	Breast	+	-	-
Du4475	Breast	+	+	+
HCC1937	Breast	+	-	-
ZR-751	Breast	+	-	-
MDA-MB231	Breast	+	-	-
BxPC-3	Pancreatic	+	-	-
L3.6pl	Pancreatic	+	-	-
Panc-1	Pancreatic	+	-	+
MCF-7	Breast	-	-	-

*The mRNA isolation and RT-PCR analysis were performed as detailed in Materials and Methods. Cell lines expressing mRNA transcripts of RON and its variants are marked as +. Deletion and insertion were determined by comparison with published RON gene sequences [25].

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