Deletion or insertion in the first immunoglobulin-plexin-transcription (IPT) domain differentially regulates expression and tumorigenic activities of RON receptor Tyrosine Kinase

Table 2 Biochemical and Biological Differences between RON160 and RON^E5/6in

Features	Similarity and Difference*:
	RON	RON160	RON^E5/6in
mRNA in tumor cell lines	11 out of 12 in CC, BC, PC lines	4 out of 12 in CC, BC lines	4 out of 12 in CC, BC, PC lines.
First IPT unit	wild-type	exon deletion	intron retention
Protein location	cell surface	cell surface	cell surface
Activation	MSP required	constitutively active	MSP required
Response to MSP	strong	moderate	strong
Trypsin digestion	sensitive	no effect	sensitive
Generation of RONp110	upon trypsin treatment	no effect	spontaneous and trypsin treatment
Furin treatment	sensitive	sensitive	less sensitive
Intracellular degradation	sensitive	less-sensitive	highly sensitive
Induction of EMT	moderate effect	strong effect	moderate effect
Cell migration	moderate effect	highly effect	moderate effect
Transforming activity	no effect	strong effect	no effect
Colony formation	no effect	strong effect	no effect

ISSN: 1476-4598