Figure 1

Infection with H. pylori leads to LRP6 phosphorylation. A, NCI-N87 cells were infected for 0.5 h or 1 h respectively with H. pylori P1 wildtype or mutant strains lacking CagA or VirB7. Additionally, cells were infected with a H. pylori P14 strain deficient for VacA. Membrane-enriched fractions were prepared as recommended by the manufacturer (S-PEK Kit, Calbiochem) and analysed by Western blot using the indicated antibodies. Diagrams indicate the relative amounts of pLRP6 after densitometric analysis. Densitometric values for pLRP6 were normalised to LRP6 and TfR1 in each sample and depicted relative to the values of non-infected cells. B, Western blot analysis of pLRP6 in NCI-N87 cells treated with recombinant Wnt3a (50 nM) for 0.5 h. C, AGS cells were infected for 0.5 h with H. pylori P1 wildtype or mutant strains lacking CagA or VirB7. Additionally, cells were infected with a H. pylori P14 strain deficient for VacA. Western blot analysis was performed as described in A. D, Western blot analysis of pLRP6 in AGS cells treated with recombinant Wnt3a (50 nM) for 0.5 h.