Figure 4

The role of GSK-3β in degradation of β-catenin. Colorectal cancer cells were treated with various doses of MSeA alone (panel 4A) or in combination with GSK-3β inhibitor, Lithium Chloride (LiCl, panel 4B). The level of total GSK-3β, p-GSK-3β and β-catenin were determined using western blots. Treatment with MSeA alone had no effect on total GSK-3β but decreased the level of p-GSK-3β only in HT-29 (panel 4A). Treatments with LiCl increased the level of p-GSK-3β (inactive form of GSK-3β) but had no effect on β-catenin expression (panel 4B). Combination treatment of LiCl/MSeA continued to inhibit expression of β-catenin only in HCT-8 (panel 4A)