Combination treatment effect on the expression of β-catenin. Cancer cells (panel 5A) and nuclear extraction of colorectal cancer cells (panel 5B) were treated with MSeA, SN-38 and docetaxel alone or in combination. Expression of β-catenin was determined using western blots. Combination treatments of MSeA/SN-38 continued to decrease expression of β-catenin when compared with all other groups. Combination treatments of MSeA/docetaxel did not reverse the inhibitory effect of MSeA alone on expression of β-catenin (panel 5A). The active form (nuclear expression) of β-catenin was inhibited by the combination treatment of MSeA/SN-38 when compared with other group (panel 5B).