Figure 1

PEA3 and ER81 mRNA and protein expression in oesophageal adenocarcinomas. (A) Examples of TMA staining for PEA3 protein (brown stain in right hand panels) in normal epithelium and adenocarcinoma samples at × 20 magnification. H&E staining of the same samples is shown on the left. (B) Summary of TMA data for moderate-high PEA3 expression in patient samples from different tissue classes. (C) The proportion of patients with T stage or N stage disease, with high PEA3 protein expression. (D and E) Box plots of PEA3 (D) and ER81 (E) mRNA expression in oesophageal tissue taken from healthy controls, Barrett's oesophagus and oesophageal adenocarcinoma patients. Median relative expression levels of PEA3 and ER81 are indicated for each tissue type. mRNA expression is calculated relative to 18S ribosomal RNA. The box plot represents the inter-quartile range and the median value is indicate by the horizontal line. The y axes are split (indicated by dashed lines) and the high outliers are labelled by case number.