Figure 4

PEA3 controls the proliferation and invasion of OE33 oesophageal adenocarcinoma cells. (A-C) Invasion assays of Het1A (A) and OE33 (B and C) cells in the presence of siRNA directed against PEA3, MMP-1 or a non targeting siRNA (control). Assays were performed for 24 hours and the number of invading cells was compared to the average number of invading cells treated with a non targeting control (taken as 1). Data are the mean and standard deviations of the relative number of invading cells from duplicate samples in 2-3 independent experiments. Statistical significance was tested by the t test. (D and E) Comparative analysis of the relative number of adherent Het1A (D) and OE33 (E) cells grown for 96 hours in the presence of the indicated targeting or non-targeting (control) siRNA duplexes. SMARTpool siRNAs (D and E) and individual siRNA contructs A and B (E) against PEA3 were used. 2 × 10⁴ cells were seeded at day 0 (indicated as 1). The data are representative of three independent experiments, and in (D) show the mean relative cell numbers and standard deviations from two experiments.