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Figure 5 | Molecular Cancer

Figure 5

From: c-Jun NH2-terminal kinase-dependent upregulation of DR5 mediates cooperative induction of apoptosis by perifosine and TRAIL

Figure 5

Perifosine activates JNK signaling ( A ), which mediates DR5 induction by perifosine ( B and C ). A, M4e cells were treated with 10 μM perifosine for the times indicated. B, M4e cells were pre-treated with the given concentrations of SP600125 for 30 min and then co-treated with 10 μM perifosine for 12 h. C, M4e cells were transfected with control (Ctrl) or JNK siRNA for 48 h and then treated with PBS or perifosine for additional 12 h. After the aforementioned treatments, the cells were subjected to preparation of whole-cell protein lysates and subsequent detection of the indicated proteins using Western blot analysis.

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