Skip to main content

Advertisement

Figure 6 | Molecular Cancer

Figure 6

From: c-Jun NH2-terminal kinase-dependent upregulation of DR5 mediates cooperative induction of apoptosis by perifosine and TRAIL

Figure 6

Effects of NAC and other antioxidants on perifosine-induced DR4 and DR5 expression ( A -C) and on enhancement of TRAIL-induced apoptosis by perifosine ( D ). A, M4e cells were pre-treated with the given concentrations of NAC for 30 min and then co-treated with 10 μM perifosine for 12 h; B, M4e cells were pre-treated with 0.5 mM Vitamin C (Vit C), or 0.5 mM tiron for 30 min and then co-treated with 10 μM perifosine for 10 h; C, M4e cells were pre-treated with the given concentrations of GSH for 30 min and then co-treated with 10 μM perifosine for 10 h. D, M4e cells were pre-treated with 5 mM NAC for 30 min and co-treated with 5 μM perifosine, 12.5 ng/ml and the combination of perifosine and TRAIL for an additional 12 h. After the aforementioned treatments, the cells were subjected to preparation of whole-cell protein lysates and subsequent detection of the indicated proteins using Western blot analysis.

Back to article page