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Figure 1 | Molecular Cancer

Figure 1

From: Downregulation of miR-342 is associated with tamoxifen resistant breast tumors

Figure 1

Expression of miR-342 is suppressed in tamoxifen resistant breast tumor cells. (A) Total RNA was isolated from tamoxifen-sensitive (MCF-7/HER2, MCF-7/pcDNA) and tamoxifen-resistant (MCF-7/HER2Δ16, TAMR1) cell lines cultured for 48 hr in 5% CS-FBS MEM and treated for 24 hr with 100 pM 17-β-estradiol (E2) and 1 μM 4-hydroxytamoxifen (TAM). RNA was analyzed by Northern Blot using DNA probes specific for miR-342-3p (miR-342) and the 5S RNA loading control. (B,C) Total RNA was isolated from each cell line treated with vehicle or E2 alone or in combination with TAM for 24 hr and (B) miR-342 or (C) EVL expression was analyzed by qRT-PCR and normalized to actin mRNA values. Levels are relative to vehicle-treated MCF-7/pcDNA cells. Data represents the mean +/- SE of triplicate samples.

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