Dual luciferase assays demonstrated down-regulation of MT1-MMP transcription in A , OVCA 420 and SKOV-3 with stable shFBI-1 knockdown. Up-regulation of MT1-MMP transcription was found in B, OVCA 420 and SKOV-3 cells after transfection of pEGFP-FBI-1 in a dose-dependent manner (upper panel); exogenous GFP-tagged FBI-1 (GFP-FBI-1) expression was determined by Western blot analysis using anti-GFP antibody (lower panel). C, ChIP assays in OVCA 420 and SKOV-3 cell lines. Protein-DNA complexes are immunoprecipitated either with anti-FBI-1 antibody or negative control, followed by PCR with primers specific to MT1-MMP promoter sequence and agarose-gel electrophoresis for visualization. Total lysates were used as the input samples and positive control.