Figure 3
Knockdown of LDH-A increases the sensitivity of Taxol-resistant 435TR1 cells to Taxol. A, MDA-435 and 435TR1 cells were transfected with scramble siRNA (Ctr) or LDH-A siRNA. 48 hrs after siRNA transfection, cell lysates were prepared and Western blotting was performed with antibodies against LDH-A. The β-actin protein was used as a loading control. B, LDH activity was examined from lysates of MDA-435 and 435TR1 48 hrs after siRNA transfection. C and D, 24 hrs after siRNA transfection, MDA-435 and 435TR1 cells were seeded into 96-well plates at the density of 8 × 103 cells per well, and treated with Taxol (5 nM and 10 nM for MDA-435, 50 nM and 100 nM for 435TR1) for 48 hrs. Then the cell viability was detected using a MTS reagent. Data are presented as the percentage of viability inhibition measured in cells treated without Taxol. Columns, mean of three independent experiments; bars, SE. *, P < 0.05, **, P < 0.01, ***, P < 0.001.