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Figure 1 | Molecular Cancer

Figure 1

From: Bisbenzamidine derivative, pentamidine represses DNA damage response through inhibition of histone H2A acetylation

Figure 1

The inhibitory effect of pentamidine on an hMRE11 nuclease and an ATM kinase. (A) Pentamidine inhibited hMRE11 nuclease. hMRE11 nuclease assay was performed using recombinant MRN complex. Indicated amount of pentamidine is added to the reaction mixture. (B) Pentamidine inhibited ATM kinase. Normal lymphoblastoid cells (GM2184) were irradiated by 10 Gy of γ-irradiation and the kinase activity assay was performed using immuno-complex by anti-ATM antibody or control rabbit IgG from these cells. Indicated concentration of pentamidine is added to the reaction mixture. (C) Pentamidine treatment increased radiation sensitivity. HeLa cells were irradiated with indicated dose of γ-ray with or without pentamidine (0.05 mM) and the viability of their cells were analyzed by colony forming assays. (D) Pentamidine treatment induced multinuclear formation. HeLa cells were irradiated with 5 Gy of γ-ray with or without pentamidine (0.05 mM) and cell morphology was observed after 24 hours.

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