Figure 3

Sindbis vector infection induces the formation of stress granules. (A) Infection with Sindbis vector induces the formation of stress granules, which can be inhibited by siPKR transfection. MOSEC cells transfected with siPKR or siGLO and infected with SV or mock infected were processed for immunofluorescence for stress granule marker TIA-1 at 6 h.p.i. Arrows in each panel indicate stress granules. (B&C) Sindbis vector infection results in the sequestration of translation initiation factors in stress granules. MOSEC cells were infected with SV or mock infected and processed for immunofluorescence using antibodies for TIA-1 and eIF4E (B) or eIF4G (C) at 6 h.p.i. Co-localization of translation initiation factors with TIA-1 indicates that they are located in stress granules following infection. Scale bars for panels A-C indicate 10 μm.