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Figure 1 | Molecular Cancer

Figure 1

From: The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression

Figure 1

NOXA and Mcl-1 bear functional GREs. A, Schematic representation describing construction of luciferase reporter plasmids containing wt and mutant human NOXA and Mcl-1 GRE promoter fragments. The numbers above the constructs indicate the region of the promoter relative to the transcription start site. Mutated bases are underlined. A549 cells were either transfected with NOXA-GREwt or NOXA-GREmut (B), Mcl-1-GREwt or Mcl-1-GREmut (C) and TAT3 (D) luciferase reporter constructs. Cells were treated with Dex (100 nM) for 16 h and luciferase assays were performed [53]. The graphs represent relative luciferase to β-galactosidase activity and data is the average of 5 independent experiments.

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