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Figure 2 | Molecular Cancer

Figure 2

From: The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression

Figure 2

Dexamethasone regulates NOXA/Mcl-1 mRNA levels in CEM-C7-14 cells. CEM-C7-14 cell were cultured in DCC-treated media and incubated with either dexamethasone (1 μM) alone or combination of dexamethasone (1 μM) with UV irradiation or SP600125 inhibitor as described in Material and Methods and [10] for the times indicated. Cells were lysed and total mRNA was extracted as described in Materials and Methods, reverse transcribed and subjected to qRT-PCR for Mcl-1, NOXA and Bim. All results have been normalised to Rpl19, which was used as an internal control. The data shown is representative of three independent experiments.

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