Figure 4
From: The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression
Dexamethasone regulates NOXA/Mcl-1 mRNA levels in A549 cells. A549 cells were cultured in DCC-treated media and incubated as described in Figure 2 above for CEM-C7-14 cells. Cells were lysed and total mRNA was extracted as described in above and subjected to qRT-PCR for Mcl-1, NOXA and Bim. All results have been normalised to Rpl19, which was used as an internal control. The data shown is representative of three independent experiments.