Figure 5
From: The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression
Dexamethasone regulates NOXA/Mcl-1 protein levels. CEM-C7-14 (A), CEM-C1-15 (B) and A549 (C) cells were cultured in DCC-treated media and incubated with dexamethasone (1 μM) for the times indicated. Cells were lysed and total cell protein extracts were subjected to western blot analysis. Mcl-1, NOXA, Bim and GR (2F8 antibody) were detected using specific antibodies against these proteins. Actin was used as loading control.