Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Analysis of an alternative human CD133 promoter reveals the implication of Ras/ERK pathway in tumor stem-like hallmarks

Figure 3

Dominant negative Ets proteins inhibit CD133 P5 activity. A, mRNA expression of ETS1, ETS2, and ELK1 genes in Caco-2 and Fuji cells. Expression pattern of 21 Ets family genes was analyzed by semi-quantitative RT-PCR analysis, in which Fuji cells with a high level of CD133 expression (CD133high) were enriched by MACS. The expression of the other genes except for ETS1, ETS2, and ELK1 are shown in Additional file 1, Figure S3. B, Transient overexpression of dominant negative Ets2 (Ets2DN) and Elk1 (Elk1DN) in Caco-2 and Fuji cell lines. Cells were transfected with pCXN2-Flag-Ets2DN and -Elk1DN lacking a transcription activation domain. The expression was evaluated by immunoblot analysis using anti-Flag antibody. C, Effects of dominant negative Ets2 and Elk1 on P5 promoter activity. P5 reporter plasmids were co-transfected with dominant negative ETS mutants. Data are means ± s.d. of values from three independent experiments. *P < 0.01 vs. mock-transfected cells.

Back to article page

Molecular Cancer

ISSN: 1476-4598