Figure 5
From: BMI1 and Mel-18 oppositely regulate carcinogenesis and progression of gastric cancer
Mel-18 overexpression as well as BMI1 knockdown in SGC-7901 cells inhibits cell migration. A) Wound healing assays were performed by plating cells in 6-well culture dishes. Cells were scratched in the middle and then further incubated for 8 hrs and 15 hrs, and the wound widths were examined under a light microscope. The migration distance of Mel-18 overexpressed SGC-7901 cells (upper panel), as well as BMI1 knockdown cells (lower panel), was decreased compared with that of control cells. Vector and Ctrl i serve as control for Mel-18 overexpression, and BMI1 knockdown (BMI1i) respectively. B) Transwell migration assays using the Corning chamber showed that fewer number of cells migrated in SGC-7901 cells with Mel-18 overexpression or BMI1 knockdown compared with that in control (p < 0.05; left, picture of migrated cells; right, the number of migrated cells were counted and plotted).