Figure 2
From: Antineoplastic effects of an Aurora B kinase inhibitor in breast cancer
AZD1152-HQPA causes mitotic defects, aneuploidy and polyploidy in breast cancer cells. (A) HER18 cells were treated with control culture medium or 20 nM AZD1152-HQPA for 48 hours, stained with DAPI and examined using fluorescence microscopy. Interphase cells showed bi-nucleation (arrow) and micronuclei (arrow heads). Metaphase cells showed misaligned chromosomes (arrow). Anaphase cells showed misegregated chromosomes (arrow) and chromosomal bridges (arrow head). (B) Percentage of cells from A with mitotic catastrophe is plotted for vehicle and 20 nM AZD1152-HQPA treatments. (C) HER18 cells were treated with AZD1152-HQPA (100 nM) for indicated times and stained with propidium iodide prior to analysis of DNA content by flow cytometry. Gating indicates 2N (G1), 4N (G2/M) and 8N (polyploid) cells. Aneuploid cells were present between 4N and 8N after 48 h treatment. (D) Percentages of 2N, 4N and 8N cells are plotted at 0, 24 and 48 hour time points based on the data in C. (E) Number of 8N cells relative to 0 hours.