EP1 receptor is involved in PGE
-mediated migration of human chondrosarcoma cells. (A) JJ012 cells were transfected with IPTG/COX-2 expression plasmid or control vector for 24 hr followed by stimulation with IPTG (5 mM) for 24 hr, the mRNA expression of EP receptors was determined by qPCR. (B) JJ012 cells were incubated with PGE2 for 24 hr, and the mRNA expression of EP receptors was determined by qPCR. (C) Total RNA were extracted from normal cartilage (lines 1-4), chondrosarcoma patients (lines 5-8) or from chondrosarcoma cell lines (SW1353 and JJ012), and subjected to qPCR analysis for EP1 receptor. (D) JJ012 cells were 17-phenyl trinor PGE2 (3 μM), butaprost (10 μM), sulprostone (10 μM), 11-deoxy-PGE1 (10 μM) and PGE2 plus SC19220 (10 μM), and in vitro migration activity measured with the Transwell after 24 hr. (E) Cells were transfected with EP receptors siRNA for 24 hr followed by stimulation with PGE2, and in vitro migration measured with the Transwell after 24 hr. Results are expressed as the mean ± S.E. *, p < 0.05 compared with control; #, p < 0.05 compared with PGE2-treated group.