Figure 4
PLC/PKC/c-Src signaling pathway is involved in PGE 2 -mediated migration and integrin upregulation in human chondrosarcoma cells. (A) JJ012 cells were incubated with PGE2 for indicated time intervals, and p-PLCβ3, p-PKCα and p-c-Src expression was determined by Western blot analysis. (B&D) JJ012 cells were pretreated for 30 min with U73122, GF109203X or PP2. Then they were followed by stimulation with PGE2, and in vitro migration and cell surface α2β1 integrin were measured with the Transwell and flow cytometry after 24 hr. (C) Cells were transfected with PKCα or c-Src mutant and PLCβ siRNA for 24 hr followed by stimulation with PGE2, and in vitro migration measured with the Transwell after 24 hr. JJ012 cells were incubated with PGE2 for indicated time intervals, and PKCα activity was determined by the PKCα kinase assay kit. Results are expressed as the mean ± S.E. *, p < 0.05 compared with control; #, p < 0.05 compared with PGE2-treated group