Figure 6

Wound healing and cell migration assays. A. Cell motility in wound healing assay. A uniform scratch was made in 80% confluent monolayer culture of MDAH-2774 cells and the extent of closure was monitored under phase-contrast microscopy as indicated and photographed. Representative images of two independent experiments done in duplicate are shown. B. Chemotactic migration of MDAH-2774 cells through membrane in Boyden chamber. Logarithmically growing cells were trypsinized and seeded in Boyden chambers and treated with various concentrations of SFN as described in methods. Migrated cells through the membrane were stained and counted with microscopy. Results of three independent experiments were plotted.