Figure 5
From: Smoothened as a new therapeutic target for human osteosarcoma
Knock down of SMO by SMO shRNA prevents osteosarcoma growth in vitro. A, Real-time PCR revealed that SMO shRNA effectively knock down SMO mRNA. (error bar means S.D.). The comparative Ct (ΔΔCt) method was used to determine fold change in expression using ACTB. Each sample was run minimally at three concentrations in triplicate (error bar means S.D.). The experiment was triplicate with similar results. B, Growth of viable 143B cells over 4 days was slowed by SMO shRNA. The experiment was triplicate with similar results. C, Colony formation assay revealed that SMO shRNA reduced colony formation in soft agar. The experiment was triplicate with similar results. (*: P < 0.01) (Error bar means S.D.)