Figure 2
From: Identification of 5 novel genes methylated in breast and other epithelial cancers
Methylation analysis of EMILIN2. (a) The region analysed by CoBRA and sequencing is shown with CpG dinucleotides numbered and shown in bold and BstUI and TaqαI restriction sites underlined. The position of CoBRA primers is also shown, covering the region between -407 bp and +110 bp with respect to the transcription start site. (b) CoBRA results are shown with undigested (U) PCR product next to digested (D) PCR product for breast cancer cell lines and normal breast. (c) CoBRA results for primary breast tumours. (d) CoBRA results for breast tumour normal pairs. (e) Selected PCR products were cloned and sequenced. Individual alleles of each of the two samples shown are represented by a line, black and white circles represent methylated and unmethylated CpG dinucleotides respectively. The frequency of methylation within the amplified region for each sample is indicated by the methylation index (MI). (f) EMILIN2 expression in normal breast tissue and re-expression in methylated breast cancer cell line MDA-MB-231 following the addition of 5-aza-2'deoxycitidine is shown.