Figure 5
From: Identification of 5 novel genes methylated in breast and other epithelial cancers
Methylation analysis of FBLN2. (a) The region analysed by CoBRA and sequencing is shown with CpG dinucleotides numbered and shown in bold and BstUI restriction sites underlined. The position of CoBRA primers is also shown, covering the region between -274 and +317 with respect to the transcription start site. (b) CoBRA results are shown with undigested (U) PCR product next to digested (D) PCR product for breast cancer cell lines and normal breast. (c) CoBRA results for primary breast tumours. (d) CoBRA results for breast tumour normal pairs. (e) FBLN2 expression in normal breast tissue and upregulation in methylated breast cancer cell line HCC1395 following the addition of 5-aza-2'deoxycitidine is shown.