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Figure 1 | Molecular Cancer

Figure 1

From: CD40 ligand induced cytotoxicity in carcinoma cells is enhanced by inhibition of metalloproteinase cleavage and delivery via a conditionally-replicating adenovirus

Figure 1

Adenovirus-delivered CD40L directly induces growth inhibition in CD40 positive carcinoma cells. (A) Analysis of CD40L and GFP expression from RAdCD40L and RAdMock viruses. HEK 293 cells were infected with RAdMock or RAdCD40L. Two days later cell lysates were probed with anti-CD40L and anti-GFP specific antibodies. (B) Analysis of the expression of CD40 receptor. 30 μg of total protein lysate from the carcinoma cell lines EJ, AGS and HONE-1 were examined for CD40 receptor expression by Western blotting using a specific anti-CD40 antibody. β-actin expression was determined as a loading control. (C) Effect of RAdCD40L on cell viability. The cancer cell lines AGS, EJ and HONE-1 were infected with 25 or 100 MOI of either RAdMock (RAdM) or RAdCD40L (RAdL) or left without infection. Forty-eight hours later cell viability was assessed by WST-1 assay. Results are the mean of triplet samples +/- SD. Two-tailed t-test RAdMock vs. RAdL: AGS, p = 0.0011; EJ, p = 0.0034; HONE-1, p = 0.4818.

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Molecular Cancer

ISSN: 1476-4598