Figure 6

Gene expression profile of DEN injected mice at four months of age. Liver/body weight ratio (A.) and serum ALT (B.) were determined in wild type mice (wt, black bar) and JDP2-transgenic mice (tg, grey bar). The results represent the average and SEM of the indicated number of animals (n). C. The expression level of the indicated genes was determined by Real time qPCR to the indicated selected genes from cDNA derived from mRNA prepared from liver tissue of either wild type control (wt) or JDP2-transgenic (tg). The expression level of each gene was normalized by the 18s ribosomal RNA and β2-microglobulin mRNA. The mRNA derived from mice injected with DEN at four weeks of age followed by Phenobarbital treatment from eight weeks of age sacrificed at four months of age. The results represent the calculated expression ratio between JDP2-transgenic (tg) divided by the expression level of the corresponding gene in the wild type mice (wt). The average and SEM of three different experiments performed with pooled mRNA from n [tg = 4/w = 3] animals.