Liver tumors analysis for 14 days DEN protocol. A. Representative liver photos derived from either wild type control (wt) and JDP2-transgenic (tg) mice are shown. B. Liver sections stained with H&E of either wild type or JDP2-tg. C. Lung section with metastatic liver tumor derived from JDP2-tg mice is shown. D. Western blot analysis of lysate derived from eleven months old mice of either wild type (wt 1-2, lanes 5-7) or JDP2-transgenic (tg 1-2, lanes 1-4) mice. Lysate was prepared from liver (non Tm, lanes 1-2 and 5-6) and liver tumors (Tm, lanes 3-4 and 7) from the same mouse cohort (indicated by a number) of the doxycycline treated mice as shown in A. Tumors were extracted only from wild type mice #1 representing the different experimental groups. Membranes were probed with anti-JDP2 (top lane in each experimental group) and anti-α-tubulin (bottom lane in each experimental group).