Silencing of Wnt-11 reduces survival of androgen-depleted LNCP cells. (a) RT-PCR of WNT11 and GAPDH in LNCaP cell lines stably expressing control (Ct) shRNA or WNT11 shRNA (clones 4 and 11) cultured for 7 days in hormone-depleted medium. (b) Images of control shRNA or WNT11 shRNA (clone 11) LNCaP cells cultured for the indicated times in hormone-depleted medium. Scale bar 200 μm. Similar results to those shown were obtained using three independent WNT11 shRNA clones. (c) Quantitation of neurite outgrowth of control shRNA or WNT11 shRNA (clone 11) LNCaP cells cultured for 4 days in hormone-depleted medium. The data presented are the means (+/- SD) derived from counting three fields of 100 cells; * p < 0.05.