NF- κ B-targeted bortezomib inhibits malignant pleural effusion (MPE)-associated inflammation, vascular leakiness, and angiogenesis. Mice were treated as in Figure 3C-D. (A) Pleural fluid and blood inflammatory cell numbers at day 14. (B) Left: MPE Evans' blue levels determined at day 14, one hour after intravenous delivery of 0.8 mg of the dye. Right: MPE/serum protein ratio. Both Evans' blue leakage and protein ration are indicators of vascular hyperpermeability. (C) Pleural tumor tissue microvessel density assessed by immunoreactivity for factor VIII-associated protein (F8A). Summary of data (left) and representative microphotographs (right; Å = 400; scale bar = 100 μm; arrows, new vessels). MPE, malignant pleural effusion; WBC, white blood cells; mono, mononuclear cells; neu, neutrophils; hpf, high-power field. Columns, mean; bars, standard error of mean; n, sample size; P, overall probability values. #, ## and ###: P < .05, .01, and .001, respectively, compared with control.