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Figure 2 | Molecular Cancer

Figure 2

From: Targeting mTORC2 inhibits colon cancer cell proliferation in vitro and tumor formation in vivo

Figure 2

Morphological changes induced by mTORC2 knockdown in colon cancer cells. (A) Morphological effects of mTOR and Rictor downregulation in HT29 and LS174T cells. HT29 or LS174T cells were infected with lentiviruses expressing shRNAs targeting the indicated genes. Following selection with puromycin for 48 hours, cells were grown on a 6 well plate for 2 or 5 additional days, and pictures were taken under a phase-contrast microscope at × 100 magnification. Scale bar: 50 μm. (B) Effects of mTOR, rictor or raptor downregulation on HT29 and LS174T cell apoptosis. HT29 and LS174T cells were processed as in A. After 2 or 5 days of culture, equal amount of cells were harvested and apoptosis was measured by quantifying DNA fragmentation using the Cell Death Detection Elisa Plus (Roche) as described by the manufacturer. Results are represented as the mean absorbance at 405 nm ± standard deviations (n = 3).

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