Figure 4
From: Targeting mTORC2 inhibits colon cancer cell proliferation in vitro and tumor formation in vivo
Colon cancer cells require mTOR or rictor to form tumors as xenografts. LS174T cells were infected with lentiviral particles containing scramble, raptor, rictor or mTOR shRNA. Stable transfectants were selected for resistance to puromycin for 48 hours and subsequently cultured in DMEM 10% FBS for 2 days. An equal amount (1 × 106) of LS174T cells deficient for raptor, rictor, or mTOR, or expressing a scramble shRNA were harvested and injected subcutaneously into immunodeficient mice (n = 5 in each group). Tumor volumes were evaluated using calliper measurements and calculated with the formula V = π/6 × a2 × b where a is the short axis and b the long axis of the tumor. Mice bearing the scramble or the raptor shRNA xenografts were sacrificed after 20 days. Mice bearing the mTOR or the rictor shRNA xenografts were observed during 60 days. Points, average value of tumor volume. Bars, SD. Animal experiments were in accordance with the Swiss federal animal regulations and approved by the local veterinary office. *p < 0.001 comparing with cells expressing the Scramble shRNA (one-way ANOVA).