Figure 1

Elevated KDM5B expression in bladder cancer in British and Japanese patients. (A) KDM5B gene expression in normal and tumor bladder tissues in British cases. Expression levels of KDM5B were analyzed by quantitative real-time PCR, and the result is shown by box-whisker plot (median 50% boxed). Relative mRNA expression shows the value normalized by GAPDH and SDH expressions. Mann-Whitney's U- test was used for statistical analysis. (B) Expression ratio between bladder normal and tumor tissues in Japanese patients. Signal intensity for each sample was analyzed by cDNA microarray, and the expression ratio is the signal intensity in tumor divided by that in normal (1 is normal). (C) Comparison of KDM5B expression between normal and tumor bladder tissues in Japanese patients. Signal intensity of each sample was analyzed by cDNA microarray, and the result is shown by box-whisker plot (median 50% boxed). Mann-Whitney's U- test was used for statistical analysis. (D) Immunohistochemical staining of KDM5B in bladder tissues. Nonimmunized mouse IgG was used as a substitute for the primary antibody to eliminate the possibility of false-positive responses from non-specific binding of IgG or from the secondary antibody. Counterstaining was done with hematoxylin and eosin. Original magnification, ×40 and ×400.