Elevated KDM5B expression in lung cancer. (A and B) Expression ratio between normal lung and tumor (non-small cell lung cancer [NSCLC] in A and small cell lung cancer [SCLC] in B) lung tissues in Japanese patients. Signal intensity for each sample was analyzed by cDNA microarray, and the expression ratio is the signal intensity in tumor divided by that in normal (1 is normal). (C and D) Comparison of KDM5B expression between normal and tumor (NSCLC and SCLC) lung tissues. Signal intensity of each sample was analyzed by cDNA microarray, and the result is shown by box-whisker plot (median 50% boxed). Mann-Whitney's U- test was used for the statistical analysis. (E) Immunohistochemical staining of KDM5B in lung tissues. Nonimmunized mouse IgG was used as a substitute for the primary antibody to verify the possibility of false-positive responses from non-specific binding of IgG or from the secondary antibody. Counterstaining was done with hematoxylin and eosin. Original magnification, ×40 and ×400.