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Figure 5 | Molecular Cancer

Figure 5

From: Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway

Figure 5

Involvement of KDM5B in the growth of bladder and lung cancer cells. (A) Quantitative real-time PCR showing suppression of endogenous expression of KDM5B by two KDM5B-specific siRNAs (siKDM5B#1 and #2) in SW780, A549 and SBC5 cells. siEGFP and siNC were used as controls. mRNA expression levels were normalized by GAPDH and SDH expressions, and values are relative to siEGFP (siEGFP = 1). Results are the mean ± SD of three independent experiments. P-values were calculated using Student's t-test (**, P < 0.01; ***, P < 0.001). (B) Effect of KDM5B siRNA knockdown on the viability of bladder cancer cell lines (RT4 and SW780) and lung cancer cell lines (A549, LC319 and SBC5). Relative cell number shows the value normalized to siEGFP-treated cells. Results are the mean ± SD in three independent experiments. P-values were calculated using Student's t-test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (C) DNA content of SBC5 cells analyzed by FACS 72 hours after the treatment with control siRNAs and siKDM5B#1. We show representative histograms for each experiment. (D) Numerical analysis of the FACS results in C, classifying cells by cell cycle status. The proportion of cancer cells in sub-G1 phase is significantly high after treatment with siKDM5B compared to control siRNAs-treated cancer cells. Results are the mean ± SD in three independent experiments. P-values were calculated using Student's t-test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).

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