Immunostaining of Klf5 and β-catenin in intestinal adenomas derived from mutant mice. Adenomatous tissues from ApcMin, ApcMin/KRASV 12and ApcMin/KRASV 12/Klf5+/- mice were formalin-fixed, paraffin-embedded and cut into 5 μm sections. Slides were then stained with Klf5 and β-catenin antibodies after deparaffinization and antigen-retrieval. Protein expression was determined upon secondary antibody treatment and color development using DAB chromogen (brown stain). Nuclei were then counterstained blue using hematoxylin. Panels A & D represent Klf5 and β-catenin staining in ApcMintumor tissues. Panels B & E represent comparative staining in ApcMin/KRASV 12tumor tissues, while panels C & F represent staining in ApcMin/KRASV 12/Klf5+/- tumor tissues.