Figure 9

Immunostaining of Klf5 and β-catenin in intestinal adenomas derived from mutant mice. Adenomatous tissues from Apc^Min, Apc^Min/KRAS^{V 12}and Apc^Min/KRAS^{V 12}/Klf5^+/- mice were formalin-fixed, paraffin-embedded and cut into 5 μm sections. Slides were then stained with Klf5 and β-catenin antibodies after deparaffinization and antigen-retrieval. Protein expression was determined upon secondary antibody treatment and color development using DAB chromogen (brown stain). Nuclei were then counterstained blue using hematoxylin. Panels A & D represent Klf5 and β-catenin staining in Apc^Mintumor tissues. Panels B & E represent comparative staining in Apc^Min/KRAS^{V 12}tumor tissues, while panels C & F represent staining in Apc^Min/KRAS^{V 12}/Klf5^+/- tumor tissues.