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Figure 6 | Molecular Cancer

Figure 6

From: Uncoupled responses of Smad4-deficient cancer cells to TNFα result in secretion of monomeric laminin-γ2

Figure 6

Analysis of regulatory sites involved in TNFα responses of the LAMA3 promoter by transient transfection of promoter-reporter constructs. Normalized promoter activities of LAMA3 wild-type and mutated promoter constructs. SW480 cells were plated in 96-well plates and transfected with the indicated promoter constructs using the Dual-Luciferase-Reporter Assay System (Promega). An NF-κB site is involved in promoter responses in Smad4-positive but not in Smad4-negative cells. An AP1 site is involved in TNFα responsiveness in a Smad4-independent manner. Bars show the mean value of three approaches with the standard error of the mean. Statistical analysis was carried out by t test (one-tailed, GraphPad Prism 4.00). *P < 0.05, **P < 0.01

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