Levels of L1-EDSBs. The figures show the number of L1-EDSB genomes per genome digested with Eco RV and Alu I and ligated to the linkers or the number of L1-EDSB genomes per control genome. (A) Duplicates or triplicates of L1-EDSB quantification from different passages and incubation times in serum-free media. Each dot of the same experiment (exp) marks HeLa cells from the same passage but derived from different tissue culture flasks. Dots within the same drawing mark cells from different experiments but whose DNA and PCR experiments were prepared simultaneously. (B) L1-EDSBs incubated for different amounts of time, 24, 48 and 72 hrs, in serum-free media.