γH2AX-bound LINE-1s in cells treated with TSA or left untreated. (A) γ-H2AX-bound LINE-1 genomes per cell treated with TSA and per control cell. (B) Correlation between the increased levels of γ-H2AX-bound L1s and L1-EDSB of control. ΔγH2AX-bound-L1s were increased γ-H2AX-bound L1s levels, calculated by the levels of TSA minus control, and L1-EDSB of controls were L1-EDSB genomes per control genome of HeLa prior to TSA treatment. (C) Methylation levels of γH2AX-bound LINE-1s of control, TSA-treated HeLa and increased γH2AX-bound DNA after TSA treatment (ΔγH2AX). Percent methylation of ΔγH2AX was calculated using the following formula: ((%methylation X γH2AX-bound LINE-1s of TSA) - (%methylation X γH2AX-bound LINE-1s of control))/((γH2AX-bound LINE-1s of TSA) - (γH2AX-bound LINE-1s of control)). The control group was comprised of cells treated with solvent lacking TSA. Data represent means ± SEM.