Migration of MDA-MB-231 and MCF-7 cells. MDA-MB-231 cells were subjected to wound-healing assay in presence or absence of PKC inhibitors (A) or after transfection with siRNA targeting PKCα (α) or a control oligonucleotide (c) (B). PKCα was downregulated in MCF-7 cells (C and D) before wound-healing assay was performed (E). Data (mean ± SEM, n = 4) show wound closure compared to closure in control conditions. Asterisks indicate statistically significant differences (* p < 0.05 and ** p < 0.01) compared to control conditions.