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Figure 1 | Molecular Cancer

Figure 1

From: Targeting Hsp90 with small molecule inhibitors induces the over-expression of the anti-apoptotic molecule, survivin, in human A549, HONE-1 and HT-29 cancer cells

Figure 1

Inhibition of Hsp90 with small molecule inhibitors induces survivin over-expression in human A549 cancer cells. (A) Concentration-dependent down-regulation of survivin in drug-treated human HeLa cancer cells. HeLa cells were treated with DMSO or various concentrations (100 nM, 250 nM and 500 nM) of 17-AAG and geldanamycin for 24 hours. Cell lysate was prepared after the treatment and various proteins were resolved by SDS-PAGE. (B) Concentration-dependent over-expression of survivin in drug-treated human A549 cancer cells. A549 cells were treated with DMSO or various concentrations (100 nM, 250 nM and 500 nM) of 17-AAG and geldanamycin for 24 hours. Cell lysate was prepared after the treatment and various proteins were resolved by SDS-PAGE. The expression of Hsp90, Akt, phosphorylated-Erk and survivin was revealed by Western blot analysis. Actin was used as an internal control. Relative ratios of expression were shown. (C) Time-dependent over-expression of survivin in drug-treated human A549 cancer cells. Human A549 cells were treated with 500 nM of 17-AAG for various intervals. Cell lysate was prepared after the treatment and various proteins were resolved by SDS-PAGE. The expression of survivin was revealed by Western blot analysis. Actin was used as an internal control. (D) Over-expression of survivin in human A549 cells treated with high concentrations of 17-AAG. A549 cells were treated with 1-2 μM of 17-AAG for 24 hours. Cell lysate was extracted after the treatment and various proteins were resolved by SDS-PAGE. Relative ratios of expression were shown.

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